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Villa, P., Dalla Vecchia, A., Piaser, E., Bolpagni, R.
Leaf reflectance and traits (pigments, structure, chemistry) collected along a gradient of water affinity, from aquatic to terrestrial species, in Mantua lakes sytem (Italy) covering different phenological stages
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Dataset connected to article:
Villa, P., Dalla Vecchia, A., Piaser, E., Bolpagni, R. (2024). Assessing PROSPECT performance on aquatic plant leaves. Remote Sensing of Environment 301, 113926.  https://doi.org/10.1016/j.rse.2023.113926

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Data Descriptions:


Field name				Description
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ID					unique sample ID that can be used to join traits and spectra data 
code					leaf sample code relating to in situ plot where leaves were collected and measured
USDAsymbol				USDA code for macrophyte species sampled
group					community type along the aquatic-terrestrial gradient (Hydr=hydrophytes (floating and emergent aquatic vegetation); Ripar=riparian vegetation; Terr=terrestrial vegetation)
year					year of sampling (2021)
month					month of sampling (June=early vegetative season; July=peak of season)


Pigments*				Description
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Chl-a					Chl-a on leaf area basis (µg cm-2)
Chl-b					Chl-b on leaf area basis (µg cm-2)
Car					total carotenoids on leaf area basis (µg cm-2)
Chl-a_Chl-b				Chl-a to Chl-b ratio
Chl_Car					Chlorophylls to Carotenoids ratio

*derived from spectrophotometric readings of absorbance of leaf extracts in acetone 80%


Structure				Description
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Thickness**				leaf blade thickness (µm)	
EWT					equivalent water thickness, or water content on leaf area basis (g cm-2)
LMA					leaf mass per area (g cm-2)
DMC					dry matter content on leaf weight (g g-1)

**derived from thin sections obseved with an optical microscope


Chemistry***				Description
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LNC					Nitrogen content on dry weight basis (% dw)
LCC					Carbon content on dry weight basis (% dw)

***derived from combustion analysis using an elemental analyser


Spectra				
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Leaf spectra were measured using a portable full range spectroradiometer (SR-3500, Spectral Evolution, Lawrence, USA) with a contact probe. During measurements, leaves were placed on a flat neoprene plate (reflectance < 5%) to minimise background reflection of light transmitted through the leaves. Leaf spectra were calibrated to reflectance using reflected radiance from a Spectralon panel. Reflectance spectra were resampled from the original SR-3500 bands (1024) to a resolution of 1 nm and truncated to a 400-2500 nm interval using cubic spline interpolation.